| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 7448 | P04004 | E. coli | ~400aa | 46kDa | 6X His |
| About this protein | |
| Name | BeyoEmbryo™ Human Vitronectin (胚胎细胞培养级)；BeyoEmbryo™ Human Vitronectin (Embryo cell culture grade)；BeyoEmbryo™ Human Vitronectin (Embryo cell culture tested)；Recombinant Human Vitronectin; 重组人玻连蛋白 |
| Synonyms | VN; V75; VNT; VTN-N; rh-VTN; rhVTN; Vitronectin; S-Protein; Serum-Spreading Factor; Epibolin |
| Source | Recombinant protein containing the truncated human vitronectin (62-478aa) is expressed in E. coli. |
| Concentration | 1mg/ml |
| Purity | Sterile, ≥ 95% by SDS-PAGE |
| Biological Activity | Used as a substrate for the feeder-free culture of human pluripotent stem cells (PSCs) and has been proven to maintain pluripotency and normal growth characteristics in multiple PSC lines such as H9 human embryonic stem cells. Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to Vitronectin-coated plates (10μg/ml and 100μl/well), approximately >40% cells will adhere specifically in 30 minutes at 37℃. |
| Physical Appearance | Liquid |
| Formulation | Liquid in 10mM Sodium Phosphate, 150mM NaCl, 0.1mM DTT, 0.1mM PMSF and 50% Glycerol, pH7.1 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in ultrapure water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Extracellular Matrix (ECM), Cell Culture, Embryo cell culture |
| Background | Vitronectin has well-defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to a variety of macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration [1,2]. |
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图1。
图1.碧云天BeyoEmbryo™ Human Vitronectin (C0318)的SDS-PAGE电泳检测效果图。本产品经BeyoGel™ Plus PAGE预制胶(Tris-Gly, 12%, 15孔) (P0459)电泳，Marker为BeyoColor™彩色预染分子量标准(10-170kDa) (P0075/P0076/P0077)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
本产品经过过滤除菌处理，可以直接稀释后用于细胞培养器皿的铺被(Coating)。
本产品可以用于无饲养层细胞条件下人胚胎干细胞或多潜能干细胞的正常培养和干性维持。本产品用于培养H9人胚胎干细胞的细胞形态和生长状态参考图2。
图2.碧云天BeyoEmbryo™ Human Vitronectin (C0318)用于培养H9人胚胎干细胞的细胞形态和生长状态效果图。使用本产品或T公司(Competitor)的Recombinant Human Vitronectin (3μg/cm2铺板)培养H9人胚胎干细胞，并观察细胞形态和生长状况。如图所示，本产品与T公司的产品相比，具有类似维持和促进H9人胚胎干细胞生长的效果。Scale Bar均为50µm。实际检测效果会因细胞、检测仪器和实验条件的不同而存在差异，本图仅供参考。
本产品培养H9人胚胎干细胞的干性维持效果通过OCT4抗体和SOX2抗体进行免疫荧光染色的效果参考图3。
图3.碧云天BeyoEmbryo™ Human Vitronectin (C0318)培养H9人胚胎干细胞的干性维持效果使用OCT4抗体和SOX2抗体进行免疫荧光染色的效果图。使用本产品或T公司(Competitor)的Recombinant Human Vitronectin (3μg/cm2铺板)培养H9人胚胎干细胞，并使用碧云天生产的C3261 多能干细胞免疫荧光检测试剂盒(Oct4, 红色荧光)和C3266 多能干细胞免疫荧光检测试剂盒(Sox2, 红色荧光)进行免疫荧光染色检测。图A、E、I为明场下H9人胚胎干细胞形态图，图B、F、J为DAPI染色效果图，图C、G、K为OCT4 (左图)和SOX2 (右图)免疫荧光染色效果图，图D、H、L为叠加效果图。如图所示，与阴性对照(Control)相比，BeyoEmbryo™ Human Vitronectin能够显著促进H9人胚胎干细胞的生长和干性维持。本产品与T公司的产品相比，具有类似维持和促进H9人胚胎干细胞生长和干性维持的效果。Scale Bar均为50µm。实际检测效果会因细胞、检测仪器和实验条件的不同而存在差异，本图仅供参考。
本产品培养H9人胚胎干细胞的细胞活力检测参考图4。
图4.碧云天BeyoEmbryo™ Human Vitronectin (C0318)培养H9人胚胎干细胞的细胞活力检测效果图。使用本产品或T公司(Competitor)的Recombinant Human Vitronectin (1或3μg/cm2铺板)培养H9人胚胎干细胞，并使用CCK-8 (C0037)检测细胞活力。如图所示，本产品与T公司的产品相比，具有类似维持和促进H9人胚胎干细胞生长的效果。实际检测效果会因细胞、检测仪器和实验条件的不同而存在差异，本图仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| C0318-500μg | BeyoEmbryo™ Human Vitronectin (1mg/ml) | 500μl |
| C0318-2mg | BeyoEmbryo™ Human Vitronectin (1mg/ml) | 2ml |
| C0318-10mg | BeyoEmbryo™ Human Vitronectin (1mg/ml) | 10ml |
| — | 说明书 | 1份 |
-20℃保存，至少一年有效。
注意事项：本产品已经过滤除菌处理，如果用于细胞培养实验，在配制Vitronectin储存液或工作液时请注意无菌操作。
为避免微量蛋白被离心管壁非特异性吸附，建议使用产品简介中Reconstitution一栏推荐的含有适量BSA的溶液配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| Plates or Dishes | Growth Area | Volume |
| 12-well plate | 3.8 cm2/well | 0.38ml/well |
| 6-well plate | 9.5 cm2/well | 0.95ml/well |
| 3.5mm dish | 9 cm2/dish | 0.9ml/dish |
| 60mm dish | 21 cm2/dish | 2.1ml/dish |
| 100mm dish | 55 cm2/dish | 5.5ml/dish |
