山羊血清
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产品编号 ： C0265
数量 ：
¥ 89.00 元
产品包装:
50ml
产品简介
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本山羊血清(Goat Serum)是碧云天自产的血清，可以用于细胞培养、免疫荧光和免疫组化时的封闭等。
本山羊血清采自健康山羊，经无菌采集、批量混合，最终过3次0.1μm过滤分装而成。所有操作符合2010版GMP (Good
Manufacturing Practices for Drug)生产标准。
本产品无细菌、真菌、支原体及病毒污染，具有高质量和高稳定性。
本山羊血清为纯天然制品，不含任何人为的添加成分，适用于科研或诊断试剂生产用。可以替代FBS用于大多数细胞系和原代细胞培养，也可用于病毒的培养和研究、鱼类细胞的培养、免疫反应中的封闭和稀释液的制备。
不同血清产品的比较、选择和使用技巧，请参考http://www.beyotime.com/support/serum.htm。
本产品通过了碧云天的细胞培养和免疫染色(包括免疫荧光和免疫组化)封闭效果测试。
包装清单：

产品编号 产品名称 包装
C0265 山羊血清 50ml
— 说明书 1份

保存条件：
-15 ~ -40℃保存，5年有效；4℃保存通常不宜超过1个月。
注意事项： 
如果不能短期内使用完毕，解冻后请适当分装。血清结冰时体积会增加约10%，因此在分装血清时须使分装瓶预留一定体积空间，否则易导致分装瓶冻裂而发生污染。
热灭活是指56℃，30分钟加热已完全解冻的血清。加热过程中需有规则地摇晃均匀。热处理的目的是灭活血清中的补体(complement)。除非必须，一般不推荐对血清进行热处理。因为热处理会造成血清沉淀物显著增多，而且还会影响血清的质量。补体参与的反应有：细胞毒作用、平滑肌细胞收缩、肥大细胞和血小板释放组胺、增强吞噬作用、促进淋巴细胞和巨噬细胞发生化学趋化和活化等。
瓶装血清解冻需采用缓慢解冻法：把在-15 ~ -40℃低温冰箱中保存的血清放入4℃冰箱中解冻约1天，待全部解冻后再分装。在解冻过程中每隔约2小时可轻轻摇晃均匀(尽量避免产生气泡)，使温度与成分均一，减少沉淀的发生。切勿直接将血清从-20℃或更低温度进入37℃水浴解冻，这样因温度改变太大，容易造成蛋白质凝集而出现沉淀，从而使用血清的质量下降。
血清中的絮状沉淀物主要是解冻后血清中纤维蛋白及4℃长时间保存后血清中的脂蛋白变性造成的，这些絮状物不会影响血清本身的质量，可不用处理。如果必须要处理，可400g离心5分钟去除。但不宜过滤去除，因为絮状物可能阻塞滤膜。
经过热处理的血清中沉淀物会显著增多。有些沉淀物在显微镜下观察像“小黑点”，而且由于这些“小黑点”的布朗运动在显微镜下被放大，感觉是在游动，所以经常被误认为血清被微生物污染。通常这些小黑点不会影响细胞生长，但如果怀疑血清存在微生物污染，则应立即停止使用，更换另一批次的血清。可将适量血清用培养液稀释至10%浓度后培养1-3天，观察小黑点是否急剧增多，或取适量血清在LB平板上涂板培养观察是否产生菌落，以确定是否存在微生物污染。
请勿将血清在37℃放置过长时间，否则血清会逐渐变浑浊，同时血清中的有效成分也会逐渐失活而影响血清质量。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

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柠檬酸钠抗原修复液(50X)
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ST023-50g
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BSA, Fraction V
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内源性过氧化物酶封闭液
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4%多聚甲醛固定液
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