结晶紫染色液
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产品编号 ： C0121-500ml
数量 ：
¥ 351.00 元
产品包装:
100ml 500ml
产品简介
使用说明
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产品问答

碧云天生产的结晶紫染色液(Crystal Violet Staining Solution)是一种组织或细胞染色时常用的可以把细胞核染成深紫色的染色液。

结晶紫是一种碱性染料，可以和细胞核中的DNA结合，从而产生细胞核染色。

一个100ml包装的本染色液至少可以染色200个样品，500ml包装的本染色液至少可以染色1000个样品。

包装清单：
产品编号 产品名称 包装
C0121-100ml 结晶紫染色液 100ml
C0121-500ml 结晶紫染色液 500ml
－ 说明书 1份
保存条件：

室温避光保存，一年有效。

注意事项：

需自备4%多聚甲醛。如果需要脱水、透明和封片处理，还需自备二甲苯，中性树胶或其它封片剂。如果样品是石蜡切片，需自备70%和90%乙醇，无水乙醇以及二甲苯。

样品数量较多时，可以使用碧云天生产的染色架和染色缸，便于操作。

第一次使用本试剂盒时建议先取1-2个样品做预实验。

结晶紫对人体有害，操作时请小心，并注意有效防护以避免直接接触人体或吸入体内。

本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。

为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.样品处理
a.对于石蜡切片：
二甲苯中脱蜡5-10分钟。
换用新鲜的二甲苯，再脱蜡5-10分钟。
无水乙醇5分钟。
90%乙醇2分钟。
70%乙醇2分钟。
蒸馏水2分钟。
b.对于冰冻切片：
蒸馏水2分钟。
c.对于培养细胞：
用4%多聚甲醛固定10分钟以上。
蒸馏水洗涤2分钟。
换用新鲜的蒸馏水，再洗涤2分钟。
2.结晶紫染色
对于上述处理好的样品：
结晶紫染色液染色10分钟(可以根据染色结果和要求调整时间)。
用蒸馏水或自来水充分洗涤后即可进行观察和拍照。
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