尼氏(Nissl)染色液
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产品编号 ： C0117
数量 ：
¥ 225.00 元
产品包装:
100ml
产品简介
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碧云天生产的尼氏(Nissl)染色液(Nissl Staining Solution)是神经生物学家广泛使用的一种Nissl染色液，用于石蜡或冰冻切片神经元细胞浆中的尼氏小体(Nissl body)染色。
Nissl染色是以德国的精神病学家和神经病理学家Franz Nissl的名字命名的。
Nissl染色液染色后呈蓝紫色，常用于显示脑或脊髓的基本神经结构。Nissl小体大而数量多，说明神经细胞合成蛋白质的功能较强；相反在神经细胞受到损伤时，Nissl小体的数量会减少甚至消失。
本Nissl染色液染色的有效成分是Cresyl violet。Cresyl violet可以和RNA或DNA结合，可以染色粗面型内质网上的核糖体，也可以染色细胞核。染色后使细胞体呈现斑驳的(mottled)蓝紫色染色。
一个包装的本染色液至少可以染色200个样品。
包装清单：

产品编号 产品名称 包装
C0117 尼氏(Nissl)染色液 100ml
— 说明书 1份

保存条件：
室温避光保存，至少一年有效。
注意事项：
特别注意：Nissl染色液的染色能力很强，并且染色后很难去除，请注意勿使染色液沾染皮肤和衣物等。
需自备4%多聚甲醛、70%乙醇和95%乙醇。如果需要脱水、透明和封片处理，还需自备二甲苯，中性树胶或其它封片剂。如果样品是石蜡切片，需自备90%乙醇，无水乙醇以及二甲苯。
样品数量较多时，可以使用碧云天生产的染色架和染色缸，便于操作。
第一次使用本试剂盒时建议先取1-2个样品做预实验。
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。


使用说明：
1. 样品处理
a. 对于石蜡切片：
二甲苯中脱蜡5-10分钟，共三次。注：脱蜡不充分会导致染色不均匀。
无水乙醇5分钟。
90%乙醇2分钟。
70%乙醇2分钟。
蒸馏水2分钟。
b. 对于冰冻切片：
用4%多聚甲醛固定10分钟以上。
蒸馏水洗涤2分钟。
换用新鲜的蒸馏水，再洗涤2分钟。
c. 对于培养细胞：
用4%多聚甲醛固定10分钟以上。
蒸馏水洗涤2分钟。
换用新鲜的蒸馏水，再洗涤2分钟。
2. 尼氏(Nissl)染色
对于上述处理好的样品：
尼氏(Nissl)染色液染色染色3-10分钟(可以根据染色结果和要求调整时间，染色时温度提高到37-50℃对于25-50微米等较厚的切片可以使染色更均匀）。
蒸馏水洗涤2次(每次数秒钟即可)。
95%乙醇约5秒。
此时，如果需要直接观察，可以用70%乙醇洗涤2次。如需脱水、透明后封片按后续步骤进行，70%乙醇洗涤后仍可按照后续步骤进行脱水、透明和封片处理。
注：如果用于免疫组化等染色后的复染，可以参考上述步骤在其它染色完成后直接进行尼氏(Nissl)染色。
3. 脱水、透明、封片或进行其它染色
a. 脱水、透明、封片：
95%乙醇脱水2分钟。
换用新鲜的95%乙醇再脱水2分钟。
二甲苯透明5分钟。
换用新鲜的二甲苯，再透明5分钟。
用中性树胶或其它封片剂封片。
显微镜下观察，细胞呈现斑驳的蓝紫色染色。
b. 进行其它染色：
如果进行免疫荧光染色，或进行Hoechst等荧光染料的染色，在Nissl染色液染色后：
70%乙醇洗涤2次，每次2分钟。
PBS或生理盐水或TBS或TBST等用于免疫染色或荧光染料染色的溶液浸泡5分钟。
然后就可以进行免疫荧光染色或其它荧光染料的染色了。
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C0105S
>200次
苏木素伊红(HE)染色试剂盒
C0105M
>1000次
苏木素伊红(HE)染色试剂盒
C1086
20次
一步法TUNEL细胞凋亡检测试剂盒(绿色荧光)
C0121-100ml
100ml
结晶紫染色液
C0115
100ml
甲基绿染色液
C1091
20次
TUNEL细胞凋亡检测试剂盒(显色法)
C0119
100ml
甲基绿-派洛宁染色液
C0602
>100次
细胞衰老β-半乳糖苷酶染色试剂盒
C0107-100ml
100ml
苏木素染色液
C1088
50次
一步法TUNEL细胞凋亡检测试剂盒(绿色荧光)
P0013B
100ml
RIPA裂解液(强)
C1005
10ml
DAPI染色液