来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
Rabbit | IHC, WB | Human | 52kDa | 52kDa |
IHC-P, Immunohistochemistry-paraffin; IHC-Fr, Immunohistochemistry-frozen; IF, Immunofluorescence;
ICC, Immunocytochemistry; WB, Western blot; FC, Flow Cytometry.
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
IHC-P | IHC-Fr | IF/ICC | WB | FC |
1:100-1:200 | N/D (not determined) | N/D | 1:500-1:2000 | N/D |
BeyoIHC™系列抗体是经过测试适用于免疫组织化学(Immunohistochemistry, IHC)的高品质抗体,也称病理抗体,很大一部分为重组兔单克隆抗体(Recombinant Rabbit Monoclonal Antibody),通过先进的兔单克隆抗体技术平台研发并生产,无需获取动物的脾脏,仅需分离抗原特异性B细胞并通过基因工程方法克隆抗体基因,然后在哺乳动物细胞中重组表达并最终纯化获得[1-2]。
BeyoIHC™抗体灵敏度更高,背景更低,染色更清晰,定位更精准。BeyoIHC™系列抗体通过反复的筛选和测试,确保灵敏度高、背景低、染色效果好[3]。
BeyoIHC™抗体特异性更强,染色结果更为准确。BeyoIHC™系列抗体大部分都是精心筛选的高效价单克隆抗体,是由单个B细胞淋巴瘤表达,只针对一个抗原表位发生特定的免疫反应,应用于免疫组化时特异性更强,染色结果更为准确[3]。
BeyoIHC™抗体一致性更好,批次间差异更小,结果重复性更好。BeyoIHC™系列抗体很大一部分是重组抗体,具有确定的抗体序列,使用不含任何动物源性物质培养液的哺乳细胞表达体系,生产的可重复性、一致性高,批次间差异小,可避免杂交瘤细胞制备过程中的基因丢失、基因突变和细胞株漂移等问题,所制备的重组抗体的重复性更好[3]。
BeyoIHC™系列抗体主要用于福尔马林固定石蜡包埋(Formalin fixed paraffin embedded, FFPE)样品的免疫组织化学染色(Immunohistochemistry-paraffin or Immunohistochemistry paraffin-embedded, IHC-P)。对于冰冻切片免疫组织化学(Immunohistochemistry frozen sections, IHC-Fr)、细胞免疫荧光(Immunofluorescence, IF)或免疫细胞化学(Immunocytochemistry, ICC)未经测试(Not determined)。同时,BeyoIHC™系列抗体可识别人的相应蛋白,对于小鼠等其它种属很大一部分未经测试。对于未经测试的应用及种属样品,理论上有可能是可以检测的,但须用户自行测试。
BeyoIHC™系列抗体配套提供了免疫染色一抗稀释液,可以用于IHC检测或其它适当用途时的一抗稀释。为进一步提高信噪比,推荐使用QuickBlock™免疫染色一抗稀释液(P0262)、SignalUp™免疫染色一抗稀释液(P0277)用于IHC检测。
抗体详细信息如下:
About this Antibody | |
Name | BeyoIHC™ SF-1 Rabbit Monoclonal Antibody |
Category | Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; IHC抗体 |
Isotype | IgG |
Clone number | BA1267 |
Purification method | Protein A affinity purification |
Positive samples | - |
Cellular location | Nucleus |
Customer validation | - |
Epitope retrieval | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0). |
About the Immunogen | |
Immunogen | Full length protein corresponding to Human SF-1. |
Sequence | - |
Gene ID | 2516 |
SwissProt | Q13285 |
Synonyms | AD4BP; ELP; FTZ1; FTZF1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3; hSF-1; NR5A1 |
Category | Transcription Factors |
Background | The orphan nuclear receptor, steroidogenic factor 1 (STF-1, also called Ad4BP), is encoded by the NR5A1 gene and plays an instrumental role in directing the transcriptional control of steroidogenesis. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that STF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render STF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of STF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma. Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an amino-terminal zinc finger DNA-binding domain, a ligand-binding domain, a carboxy-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function, acetylation enhances its transcriptional activity. |
产品编号 | 产品名称 | 包装 |
AG8684 | BeyoIHC™ SF-1 Rabbit Monoclonal Antibody | 50µl |
AZ060 | 免疫染色一抗稀释液 | 10ml |
— | 说明书 | 1份 |
-20℃保存,一年有效。免疫染色一抗稀释液也可以4℃保存,长期不使用推荐-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:如果本抗体用于免疫组化(IHC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常可以重复使用1-5次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000×g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的免疫染色一抗稀释液也可以用于Western blot (WB)等适当用途。如果希望获得最佳的检测效果,推荐使用Western一抗稀释液(P0023)。
抗原修复过程中应防止缓冲液过度蒸发,切勿干片。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。