| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, FC, IP | H, R, B, C, Dg, Mk | 29kDa | 29kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; B, Bovine; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1,000-1:2,000 | 1:50 | - | - | - | 1:800-1:2,000 | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | PCNA Rabbit Monoclonal Antibody |
| Category | Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; 内参抗体 |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | - |
| Cellular location | - |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | A synthetic peptide corresponding to residues aa100-200 of human PCNA was used as an immunogen |
| Sequence | - |
| Gene ID | 5111 |
| SwissProt | P12004 |
| Synonyms | ATLD2; PCNA |
| Category | Cell Cycle; ErbB/HER Signaling |
| Background | Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation. |
| 产品编号 | 产品名称 | 包装 |
| AG8029 | PCNA Rabbit Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
PCNA Rabbit Monoclonal Antibody -20℃保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
All lanes: Anti-PCNA antibody at 1:2,000 dilution
Predicted MW: 29 kDa
Observed MW: 29 kDa
Lane 1: A431
Lane 2: 293
Lane 3: Hela
Lane 4: HepG2
Lane 5: MCF-7
Lane 6: 3T3
Lane 7: PC-12
Lysates at 10 μg per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 10s
PCNA was immunoprecipitated from 0.4mg of HeLa whole cell lysate with PCNA Rabbit Monoclonal Antibody at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: PCNA Rabbit Monoclonal Antibody IP in HeLa whole cell lysate
Lane 2: PBS instead of PCNA Rabbit Monoclonal Antibody in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10μg (input)
Exposure: 20s
Overlay histogram showing HeLa cells stained with PCNA Rabbit Monoclonal Antibody (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (PCNA Rabbit Monoclonal Antibody, 1:2,000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
