Mono-Methyl-Histone H3 (Lys27) Rabbit Polyclonal Antibody
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产品编号 AF5641
数量
¥ 593.00
产品包装:
50μl
产品简介
使用说明
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来源 用途 交叉反应性 理论分子量 实际分子量
Rabbit WB, IP, IF, IHC, ChIP, ChIPseq H, M, R, Other (Wide Range) 15kDa 18kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:200 - 1:20-1:100 1:20-1:100

抗体详细信息如下::

About this Antibody
Name Mono-Methyl-Histone H3 (Lys27) Rabbit Polyclonal Antibody
Category Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype IgG
Purification method Affinity purification
Positive samples HeLa, H3 protein
Cellular location Chromosome, Nucleus
Customer validation WB (Human)
About the Immunogen
Immunogen A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
Sequence -
Gene ID 8290
Swiss Prot Q16695
Synonyms HIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t
Category Histone Methylation; Crosstalk Between Protein Modifications; Epigenetics; MAPK/Erk in Growth & Differentiation
Background The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker.

包装清单:

产品编号 产品名称 包装
AF5641 Mono-Methyl-Histone H3 (Lys27) Rabbit Polyclonal Antibody 50μl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:
Mono-Methyl-Histone H3 (Lys27) Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:

Fig. 1. Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Fig. 2. Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody.
Fig. 3. Immunohistochemistry of paraffin-embedded rat brain using MonoMethyl-Histone H3-K27 antibody at dilution of 1:200 (40x lens). Fig. 4. Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K27 antibody. Blue: DAPI for nuclear staining.

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