SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1)
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产品编号 AF0501-50μg
数量
¥ 621.00
产品包装:
50μg 200μg 1mg
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来源 用途 交叉反应性 理论分子量 实际分子量
Human WB, ELISA SARS-CoV-2 (2019-nCoV) 48kDa ~48kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ELISA
1-5µg/ml - - - - - - 0.1-5µg/ml

抗体详细信息如下:

About this Antibody
Name SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1), SARS-CoV-2 N Protein hMAb (Clone B1)
Category Human Monoclonal Antibody (mAb); Primary Antibody
Isotype Human/IgG1
Purification method Protein A
Positive samples -
Cellular location -
Customer validation This antibody can pair with SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B2) (AF0503) as capture antibody for ELISA.
About the Immunogen
Immunogen -
Sequence -
Gene ID 43740575
Swiss Prot -
Synonyms N protein; Nucleocapsid phosphoprotein; Nucleocapsid protein; N structural protein
Category Anti-infection
Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus.

本抗体的相关数据:

Figure 1. The antibody stability at room temperature was determined by functional ELISA. Immobilized 2019-nCoV Nucleocapsid Protein (新型冠状病毒N蛋白) (P2328) at 100ng/well can bind SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1). After storing at room-temperature for 1 week, 2 weeks or 4 weeks, the EC50 of this product showed no significant change.

Figure 2. The repeated freeze-thaw (FT) stability of antibody was determined by functional ELISA. Immobilized 2019-nCoV Nucleocapsid Protein (新型冠状病毒N蛋白) (P2328) at 100ng/well can bind SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1). After 10 freeze-thaw cycles, the EC50 of this product showed no significant change.

Figure 3. Immobilized 2019-nCoV Nucleocapsid Protein (新型冠状病毒N蛋白) (P2328) at 100ng/well can bind SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1), and the EC50 is less than 0.1μg/ml.
包装清单:
产品编号 产品名称 包装
AF0501-50µg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 50µg
AF0501-200µg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 200µg
AF0501-1mg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 1mg
说明书 1份
保存条件:

SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 4℃保存,至少一年有效。短期内不使用,推荐-20℃保存,-20℃可以保存更长时间。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000×g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页: http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。
相关产品:
产品编号 产品名称 包装
AF0501-50µg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 50µg
AF0501-200µg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 200µg
AF0501-1mg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B1) 1mg
AF0503-50µg SARS-CoV-2 N Protein Human Monoclonal Antibody (Clone B2) 50µg
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