| 来源 | 用途 | 交叉反应性 | 分子量 |
| Mouse | WB, ICC, IHC, FC, IF | H, M | 130KDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:500-1:1000 | - | 1:200 | 1:200 | 1:200 | 1:50-1:100 | - | - |
抗体详细信息如下:
| About this Antibody | |
| Name | E-Cadherin Mouse Monoclonal Antibody |
| Category | Monoclonal antibody(mAb); Primary antibody |
| Isotype | IgG1 |
| Purification | ProA affinity purified |
| About the Immunogen | |
| Immunogen | This antibody is produced by immunizing mice with recombinant protein to a region of E-cadherin. |
| Gene ID | 999 |
| SwissProt | P12830 |
| Synonyms | CADH1; cadherin 1; type 1; E-cadherin (epithelial) (ECAD); cadherin-1; CAM 120/80; E-cadherin; epithelial-cadherin; uvomorulin |
| Category | Adhesion/ECM |
| Background | E-cadherin (epithelial) is the most well-studied member of the cadherin family. It consists of 5 cadherin repeats (EC1 ~ EC5) in the extracellular domain, one transmembrane domain, and an intracellular domain that binds p120-catenin and beta-catenin. The intracellular domain contains a highly-phosphorylated region vital to beta-catenin binding and, therefore, to E-cadherin function. Loss of E-cadherin function or expression has been implicated in cancer progression and metastasis. E-cadherin downregulation decreases the strength of cellular adhesion within a tissue, resulting in an increase in cellular motility. This in turn may allow cancer cells to cross the basement membrane and invade surrounding tissues. E-cadherin is also used by pathologists to diagnose different kinds of breast cancer. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF0138 | E-Cadherin Mouse Monoclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
E-Cadherin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
A. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
B. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
C. 回收稀释的一抗，4℃保存以备下次继续使用。
D. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片：
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| Fig. 1. Western blot analysis of E-cadherin on different cell lysates using anti- E-cadherin antibody at 1/1000 dilution.Positive control: Lane 1: A431 Lane 2: SW480 Lane 3: MCF-7 Lane 4: Hela. | Fig. 2. Immunocytochemistry staining E-Cadherin in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS. |
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| Fig. 3. Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using E-Cadherin antibody. Counter stained with hematoxylin. | Fig. 4. Flow cytometric analysis of HeLa cells with E-Cadherin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
