| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IF, IHC, ICC | H, M, Rb | 15KDa | 14/16KDa |
WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry. H, Human; M, Mouse; Rb, Rabbit.
BeyoAb™系列抗体为经深入测试的优质抗体，并配套提供Western一抗稀释液，可用于Western或其它适当用途的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整，NT, not tested)。
| WB | IP | IF | IHC | ICC | FC | ChIP |
| 1:1000-1:2000 | NT | 1:50-1:200 | 1:50 | 1:50-1:200 | NT | NT |
本抗体相关应用的代表性检测效果图片请参见Fig. 1。
Fig. 1. BeyoAb™ LC3B Rabbit Polyclonal Antibody (AB2023) was used for Western blot, immunofluorescence and immunohistochemistry.
A. Western analysis of lysates from indicated cell lines treated with 50μM chloroquine for 20h using BeyoAb™ LC3B Rabbit Polyclonal Antibody (AB2023) at 1:2000 dilution and BeyoWB™ HRP-labeled Goat Anti-Rabbit IgG (H+L) (P0948) at 1:500 dilution. 20µg protein per lane was loaded and analyzed by BeyoWB™ 80min Electrophoresis, Transfer and Western Blot Kit (P0002). Exposure time, 2s.
B. Immunofluorescence analysis of HeLa cells treated with 50μM chloroquine for 20h using BeyoAb™ LC3B Rabbit Polyclonal Antibody (AB2023) at a dilution of 1:100 and Cy3-labeled Goat Anti-Rabbit IgG (H+L) (A0516) at 1:250 dilution. a. Brightfield. b. Immunofluorescence image for LC3B. c. Nuclear staining using Antifade Mounting Medium with DAPI (P0131). d, Merged image for b and c.
C. Immunohistochemistry of a section from paraffin-embedded mouse brain tissue using BeyoAb™ LC3B Rabbit Polyclonal Antibody (AB2023) at a dilution of 1:50 (bottom panel) compared to a negative control without primary antibody (Top panel). HRP-labeled Goat Anti-Rabbit IgG (H+L) (A0208) was used as secondary antibody at 1:1000 dilution.
抗体详细信息如下:
| About this Antibody | |
| Name | BeyoAb™ LC3B Rabbit Polyclonal Antibody |
| Category | Polyclonal antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | HeLa; NIH/3T3 |
| Cellular location | - |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | Recombinant protein |
| Sequence | - |
| Gene ID | 81631 |
| SwissProt | Q9GZQ8 |
| Synonyms | MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta |
| Category | Organelle Markers; Autophagy Signaling |
| Background | Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents [1]. Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer. Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy [2]. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles. The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy [3]. |
| 产品编号 | 产品名称 | 包装 |
| AB2023-50μl | BeyoAb™ LC3B Rabbit Polyclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AB2023-200μl | BeyoAb™ LC3B Rabbit Polyclonal Antibody | 200μl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
-20ºC保存，至少一年有效。Western一抗稀释液也可以4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4ºC保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| AB2023 | BeyoAb™ LC3B Rabbit Polyclonal Antibody | 50μl/200µl |
| AL221 | LC3B抗体(兔多抗) | >20次 |
| AF5225 | LC3B Rabbit Polyclonal Antibody (KO Validated) | 50μl |
| P0001 | BeyoWB™ 30分钟Western检测试剂盒 | 6-15次/30-75次 |
| P0002 | BeyoWB™ 80分钟电泳转膜与Western检测试剂盒 | 6-15次/30-75次 |
| P0956 | BeyoWB™ 30分钟Western检测试剂盒(小鼠一抗用) | 6-15次/30-75次 |
| P0958 | BeyoWB™ 30分钟Western检测试剂盒(兔一抗用) | 6-15次/30-75次 |
| P0946 | BeyoWB™ HRP标记山羊抗小鼠IgG(H+L) | 200μl/1ml |
| P0948 | BeyoWB™ HRP标记山羊抗兔IgG(H+L) | 200μl/1ml |
| P0950 | BeyoWB™封闭液 | 100ml/500ml |
| P0951 | BeyoWB™抗体稀释液 | 100ml/500ml |
| P0952-500ml | BeyoWB™洗涤液 | 500ml |
| P0953-100ml | BeyoWB™洗涤液(10X) | 100ml |
| P0955 | BeyoWB™ BeyoECL | 100ml/500ml |
| E6001 | MiniProGel™蛋白制胶与电泳系统(4胶) | 1套 |
| E6050 | MiniBlot™蛋白转膜系统 | 1套 |
| E6150 | MiniProGel™蛋白制胶、电泳与转膜系统(2胶/2膜) | 1套 |
| E6155 | MiniProGel™蛋白制胶、电泳与转膜系统(4胶/2膜) | 1套 |
| E6080 | BeyoPower™中电流电源(300V/600mA/100W) | 1套 |
| E6085 | BeyoPower™高电流电源(300V/2000mA/200W) | 1套 |
| FFP24 | PVDF膜(进口分装, 6.6×8.5cm, 0.2μm) | 20张/包装 |
| FFP32 | PVDF膜(进口分装, 6.6×8.5cm, 0.45μm) | 20张/包装 |
| FFP51 | 转印滤纸(7.5×10cm) | 100张/包装 |
| FFX050-FFX055 | Western洗膜盒 | 1个/包装 |
| P0014A/P0014B | SDS-PAGE电泳液(Tris-Gly, Powder) | 1L/10L |
| P0015/P0015L | SDS-PAGE蛋白上样缓冲液(5X) | 2ml/15ml |
| P0015F | SDS-PAGE蛋白上样缓冲液(6X) | 2ml |
| P0017F | BeyoBlue™考马斯亮蓝超快染色液 | 250ml |
| P0018A | BeyoECL Star (特超敏ECL化学发光试剂盒) | 100ml/500ml |
| P0018F | BeyoECL Moon (极超敏ECL化学发光试剂盒) | 100ml/500ml |
| P0021A | Western转膜液 | 1L |
| P0021S | PVDF膜浸润活化液 | 250ml/500ml×2 |
| P0022 | 丽春红染色液 | 120ml/500ml |
| P0023A | Western一抗稀释液 | 100ml/500ml |
| P0023B | Western封闭液 | 100ml/500ml |
| P0023C | Western洗涤液 | 250ml/500ml×2 |
| P0023D | Western二抗稀释液 | 100ml/500ml |
| P0025 | Western一抗二抗去除液(强碱性) | 250ml/1L |
| P0066 | 预染蛋白质分子量标准(19-117kD) | 200μl |
| P0068 | 彩色预染蛋白质分子量标准(10-180kD) | 200μl |
| P0071 | BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) | 200μl |
| P0075 | BeyoColor™彩色预染蛋白分子量标准(10-170kD) | 200μl |
| P0078 | BeyoColor™彩色预染蛋白分子量标准(15-120kD) | 200μl |
| P0095 | 免疫染色通透液(Saponin) | 100ml/500ml |
| P0096 | 免疫染色通透液(Triton X-100) | 100ml/500ml |
| P0097 | 免疫染色强力通透液 | 100ml/500ml |
| P0098 | 免疫染色固定液 | 100ml/500ml |
| P0099 | 4%多聚甲醛固定液 | 100ml/500ml/500ml×6 |
| P0102 | 免疫染色封闭液 | 100ml |
| P0103 | 免疫染色一抗稀释液 | 100ml |
| P0106 | 免疫染色洗涤液 | 250ml |
| P0108 | 免疫荧光染色二抗稀释液 | 100ml |
| P0220 | Quickblock™封闭液(PBS) | 100ml |
| P0235 | Quickblock™封闭液(10X) | 100ml |
| P0252 | QuickBlock™ Western封闭液 | 100ml/500ml |
| P0256 | QuickBlock™ Western一抗稀释液 | 100ml/500ml |
| P0258 | QuickBlock™ Western二抗稀释液 | 100ml/500ml |
| P0260 | QuickBlock™免疫染色封闭液 | 100ml |
| P0262 | QuickBlock™免疫染色一抗稀释液 | 100ml |
| P0265 | QuickBlock™免疫荧光染色二抗稀释液 | 100ml |
| P0267 | QuickBlock™免疫组化染色二抗稀释液 | 100ml |
| P0280 | InstantView™ SDS-PAGE蛋白染色及上样缓冲液(5X) | 1ml |
