Bcl-2抗体(兔多抗)
收藏
产品编号 AB112
数量
¥ 793.00
产品包装:
>20次
产品简介
使用说明
相关文件下载
相关产品
相关论文
产品问答
来源 用途 交叉反应性 抗体识别位点 Bcl-2分子量
Rabbit WB, IF, IHC, ICC, FC H, M Bcl-2 C-terminal ~28kD

WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry.
H, Human; M, Mouse.
本Bcl-2抗体(Bcl-2 antibody)为进口分装,用人工合成的Bcl-2羧基端(carboxy-terminal)的一段多肽进行适当修饰后免疫rabbit,然后用protein A和抗原多肽亲和柱经过两步纯化而得到的高纯度抗体。
本Bcl-2抗体可以识别内源性水平的Bcl-2,在生理水平检测时未发现和其它Bcl-2家族蛋白有交叉反应。
Bcl-2是一种抗凋亡蛋白,可以抑制很多凋亡诱导剂诱导的细胞色素c释放(cytochrome c release),及后续的细胞凋亡。Bcl-2被推测可以影响线粒体的钙离子平衡(calcium homeostasis)和质子流(proton flux)。通常Bcl-2蛋白水平的上调被认为细胞的抗凋亡能力上调,而Bcl-2蛋白水平的显著下降则可能导致细胞凋亡。
配套提供了Western一抗稀释液,可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ELISA ChIP
1:1000 - 1:100 1:250 1:100 1:150 - -

本抗体如果用于常规的Western检测至少可以检测20次。
包装清单:

产品编号 产品名称 包装
AB112-1 Bcl-2抗体(兔多抗) 20μl
AB112-2 Western一抗稀释液 20ml
说明书 1份

保存条件:
Bcl-2抗体-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. Western检测:
A. 按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。
B. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
C. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
D. 回收稀释的一抗,4℃保存以备下次继续使用。
E. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。

相关产品请点击如下按钮:
使用本产品的文献:
1. Wang W, Zeng C, Feng Y, Zhou F, Liao F, Liu Y, Feng S, Wang X
Environ Pollut 2018 Feb;233:218-225. (IF 6.792)
2. Lan Jin, Yunhe Chen, Dan Cheng, Zhikai He, Xinyi Shi, Boyu Du, Xueyan Xi, Yujing Gao, Yang Guo
Cell Death Dis. doi: 10.1038/s41419-021-03722-8. (IF 6.304)
3. Jing Zhao, Guorong Wang, Kaitao Han, Yang Wang, Lin Wang, Jinxia Gao, Sen Zhao, Gang Wang, Shengyang Chen, An Luo, Jianlin Wu, Guangzhi Wang
Cell Death Dis. doi: 10.1038/s41419-022-05051-w. (IF 6.304)
4. Juncheng Liu, Fang Wang, Zebin Weng, Xiaonan Sui, Yong Fang, Xiaozhi Tang, Xinchun Shen
Genomics. doi: 10.1016/j.ygeno.2020.05.011. (IF 6.205)
5. Zhilei Mao, Mengmeng Yao, Yaqi Li, Ziqiang Fu, Shushu Li, Lina Zhang, Zhu Zhou, Qiusha Tang, Xiumei Han, Yankai Xia
BIOMATER SCI-UK. doi: 10.1039/c8bm00856f. (IF 6.183)
6. Zhang L, Liu X, Liu J, Ma X, Zhou Z, Song Y, Cao B
J Cell Physiol 2018 Jun;233(6):4688-4706. (IF 5.546)
7. Zhang L, Liu X, Ma X, Liu Y, Che S, Cui J, An X, Cao B, Song Y
J Cell Physiol 2018 Oct;233(10):6965-6974. (IF 5.546)
8. Song F, Zhang L, Yu H, Lu R, Bao J, Tan C, Sun Z.
Food Chemistry 2012 May;132(1):43-50. (IF 5.399)
9. Xin-Can Zhao, Li Zhang, Hui-Xin Yu, Zhen Sun.
Food Chemistry 2012 Nov;129(2):387394. (IF 5.399)
10. Jin M, Zhang L, Yu H, Meng J, Sun Z, Lu R.
Food Chemistry 2013 Nov;41(2);847–52. (IF 5.399)
11. Zheng-Hao Zhang, Zi-Guan Zhang, Min-Wei Chen, Ying Yang, Run-Jing Li, Jia-Jia Xu, Cui Yang, Yu-Ying Li, Hong-Wei Chen, Shi-Xiao Liu, Yan-Ling Li, Ping Luo, Yi-Jiang Liu, Wen-Bo Chen, Zhong-Gui Shan, Zheng-Rong Huang
Oxid Med Cell Longev. doi: 10.1155/2022/1115749. (IF 5.076)
12. Liu RT, Zou LB, Lü QJ.
Acta Pharmacol Sin 2009 Jul;30(7):899-906. (IF 5.064)
13. Zhao F, He EQ, Wang L, Liu K.
J Asian Nat Prod Res 2008 May-Jun;10(5-6):467-73. (IF 1.345)
14. Sun ZQ, Deng CS, Xu SY, Du Y.
WORLD J GASTROENTERO 2008 Oct 14;14(38):5827-33. (IF 3.665)
15. K Xu, Q Ding, Z Fang, J Zheng, P Gao, Y Lu and Y Zhang.
Cancer Gene Therapy 2009:1–11. (IF 4.681)
16. Li Y, Li Q, Wang Z, Liang D, Liang S, Tang X, Guo L, Zhang R, Zhu D.
Apoptosis 2009 Jan;14(1):42-51. (IF 4.543)
17. Zhang M, Zhang H, Sun C, Shan X, Yang X, Li-Ling J, Deng Y.
CANCER CHEMOTH PHARM 2009 Mar;63(4):635-42. (IF 2.967)
18. Xia M, Bian M, Yu Q, Liu J, Huang Y, Jin X, Lu S, Yu M, Huang F.
ACTA BIOCH BIOPH SIN 2011 Jun;43(6):448-54. (IF 2.836)
19. Gong L, Liu FQ, Wang J, Wang XP, Hou XG, Sun Y, Qin WD, Wei SJ, Zhang Y, Chen L, Zhang MX.
BBA-BIOMEMBRANES 2011 Jun;1813(6):1211-9. (IF 3.411)
21. Khan M, Ding C, Rasul A, Yi F, Li T, Gao H, Gao R, Zhong L, Zhang K, Fang X, Ma T.
Int J Biol Sci 2012;8(4):533-47. (IF 4.858)
22. Ding C, Khan M, Zheng B, Yang B, Zhong L, Ma T.
AFR J PHARM PHARMACO 2012 Feb;6(6):412-18. (IF 0.5)
23. Li Z, Pang L, Fang F, Zhang G, Zhang J, Xie M, Wang L.
Brain Research 2012 April;1450:116–24. (IF 2.929)
24. Gao Z, Kang X, Hu J, Ju Y, Xu C.
Cytotechnology 2012 Aug;64(4):421-8. (IF 1.777)
25. Muhammad Khan, Javed Iqbal Qazi, Azhar Rasul, Zheng Y, Ma T.
BANGL J PHARMACOL 2013; 8: 8-14. (IF 1.306)
26. Cao ZH, Yin WD, Zheng QY, Feng SL, Xu GL, Zhang KQ.
Cell Biochem Biophys 2013;67(3):1239-48. (IF 2.073)
27. Wu L, Xi Z, Guo R, Liu S, Yang S, Liu D, Dong S, Guo D.
Avian Pathol 2013 Feb;42(1):32-7. (IF 2.338)
28. Liu Z, Yu Y, Yuan Z, Zhang F, Jing C.
Toxin Reviews 2013 May;32(2):34-8 . (IF 3.84)
29. Sun B, Cai Y, Li Y, Li J, Liu K, Li Y, Yang Y.
Virology 2013 May 25;440(1):75-83. (IF 2.819)
30. Xia L, Huang Q, Nie D, Shi J, Gong M, Wu B, Gong P, Zhao L, Zuo H, Ju S, Chen J, Shi W.
Brain Res 2013 Jul 12;1521:68-78. (IF 2.733)
31. Li J, Xu L, Ye J, Li X, Zhang D, Liang D, Xu X, Qi M, Li C, Zhang H, Wang J, Liu Y, Zhang Y, Zhou Z, Liang X, Li J, Peng L, Zhu W, Chen YH.
J Cell Mol Med 2013 Sep;17(9):1119-27. (IF 4.486)
32. Zamani M, Soleimani M, Golab F, Mohamadzadeh F, Mehdizadeh M, Katebi M.
Metab Brain Dis 2013 Sep;28(3):367-74. (IF 2.726)
33. Bai F, Ni B, Liu M, Feng
Vet Microbiol 2015 Jan 30;175(1):58-67. (IF 3.03)
34. Zhang F, Hu Y, Xu X, Zhai X, Wang G, Ning S, Yao J, Tian X.
J Surg Res 2015 Mar;194(1):127-38. (IF 1.841)
35. Huang G, Mao J, Ji
Food Funct 2015 Mar;6(3):765-71. (IF 4.171)
37. Wang P, Peng X, Wei ZF, Wei FY, Wang W, Ma WD, Yao LP, Fu YJ, Zu YG.
BBA-BIOMEMBRANES 2015 Sep; 1850(9):1751-61. (IF 3.411)
38. Dong M, Lu Y, Zou Z, Yang
Life Sci 2015 Oct 1;138:64-71. (IF 3.647)
39. Liu MH, Lin XL, Guo DM, Zhang Y, Yuan C, Tan TP, Chen YD, Wu SJ, Ye ZF, He J.
Mol Med Rep 2016 Feb;13(2):1281-6. (IF 2.1)
40. Ding L, Wu J, Li D, Wang H, Zhu B, Lu W, Xu G.
Mol Med Rep 2016 Mar;13(3):2017-22. (IF 2.1)
41. Zhang LI, Wang C, Liu S, Zhao Y, Liu C, Guo Z.
Oncol Lett 2016 Jun;11(6):3961-6. (IF 2.311)
42. Yu XB, Chen XH, Ling F, Hao K, Wang GX, Zhu B.
ANTIVIR RES 2016 Jul;131:156-65. (IF 4.101)
43. Yao J, Xie J, Xie B, Li Y, Jiang L, Sui X, Zhou X, Pan H, Han W.
Biochem Pharmacol 2016 Sep 1;115:51-63. (IF 4.96)
44. Peng JY, Gao KX, Xin HY, Han P, Zhu GQ, Cao BY.
DOMEST ANIM ENDOCRIN 2016 Oct;57:108-16. (IF 1.778)
46. Chen P, He D, Zhang Y, Yang S, Chen L, Wang S, Zou H, Liao Z, Zhang X, Wu M.
Food Funct 2016 Nov 9;7(11):4576-88. (IF 4.171)
48. Sun XF, Li YP, Pan B, Wang YF, Li J, Shen W
Cell Cycle 2018;17(18):2230-2242. (IF 3.699)
51. Sun H, Zhu Z, Tang Y, Ren Y, Song Q, Tang Y, Zhang Y
Food Funct 2018 May 23;9(5):2744-2754. (IF 4.171)
52. Chen Y, Zhuang H, Chen X, Shi Z, Wang X
Oncol Rep 2018 Jun;39(6):2845-2854. (IF 3.417)
54. Chu M, Wang T, Sun A, Chen Y
Exp Ther Med 2018 Jul;16(1):370-376. (IF 1.785)
55. Yu F, Zhang Y, Ye L, Tang Y, Ding G, Zhang X, Yang Z
Mol Med Rep 2018 Jul;18(1):771-778. (IF 2.1)
56. Kang J, Jia Z, Ping Y, Liu Z, Yan X, Xing G, Yan W
Arch Biochem Biophys 2018 Jul 1;649:53-59. (IF 3.391)
57. Cheng SF, Qin XS, Han ZL, Sun XF, Feng YN, Yang F, Ge W, Li L, Zhao Y, De Felici M, Zou SH, Zhou Y, Shen W
AGING-US 2018 Jul 12;10(7):1556-1574. (IF 4.831)
58. Liu X, Zhang L, Liu Y, Cui J, Che S, An X, Song Y, Cao B
BBA-MOL CELL RES 2018 Aug;1865(8):1130-1147. (IF 4.105)
59. Zhai QY, Ge W, Wang JJ, Sun XF, Ma JM, Liu JC, Zhao Y, Feng YZ, Dyce PW, De Felici M, Shen W
AGING-US 2018 Aug 28;10(8):2170-2189. (IF 4.831)
60. Dong J, Geng J, Tan W
Biomed Pharmacother 2018 Sep;105:922-931. (IF 4.545)
61. Yang Y, Wu JJ, Cheng CD, Bao DJ, Dong YF, Li DX, Niu WX, Zhou CX, Niu CS
Am J Transl Res. 2018 Nov 15;10(11):3370-3384. eCollection 2018. (IF 3.375)
62. Zeng C, Feng Y, Wang W, Zhou F, Liao F, Liu Y, Feng S
Environ Toxicol 2018 Dec;33(12):1221-1228. (IF 3.118)
63. Fu B, Xi S, Wang Y, Zhai X, Wang Y, Gong Y, Xu Y, Yang J, Qiu Y, Wang J, Lu D, Huang S
Front Pharmacol 2019 Jan 8;9:1539. (IF 4.225)
64. Huan Gao, Sixi Zhang, Tingting Hu, Xiaoyu Qu, Jinghui Zhai, Yueming Zhang, Lina Tao, Jianyuan Yin, Yanqing Song
CHEM-BIOL INTERACT. doi: 10.1016/j.cbi.2018.11.008. (IF 3.723)
65. Biqian Fu, Shengyan Xi, Yanhui Wang, Xiangyang Zhai, Yanan Wang, Yuewen Gong, Yangxinzi Xu, Jiaqi Yang, Yingkun Qiu, Jing Wang, Dawei Lu, Shuqiong Huang
Front Pharmacol. doi: 10.3389/fphar.2018.01539. (IF 4.225)
66. Zhang L, Pang Y, Cui X, Jia W, Cui W, Liu Y, Liu C, Li F
Oncol Lett. 18(1):936-943. (IF 2.311)
67. Zheng J, Cheng X, Xu S, Zhang L, Pan J, Yu H, Bao J, Lu R
Food Funct. 10(11):7253-7261. (IF 4.171)
68. Li JL, Wang JP, Chang H, Deng SM, Du JH, Wang XX, Hu HJ, Li DY, Xu XB, Guo WQ, Song YH, Guo Z, Sun MX, Wu YW, Liu SB
CANCER MED-US. 8(18):7774-7780. (IF 3.491)
69. Dai J, Ma J, Liao Y, Luo X, Chen G
Braz J Med Biol Res. 52(12):e8834. (IF 2.023)
70. Anzana Parvin, Shuang-Li Hao, Fu-Qing Tan, Wan-Xi Yang
Gene. doi: 10.1016/j.gene.2020.144989. (IF 2.984)
71. Shi Chen, Shiyi Tan, Shang Yang, Gang Chen, Li Zhu, Zhiqian Sun, Haibin Li, Sanqiao Yao
Toxicol Lett. doi: 10.1016/j.toxlet.2020.09.019. (IF 3.569)
72. Shichen Xu, Xian Cheng, Liying Wu, Jiangxia Zheng, Xiaowen Wang, Jing Wu, Huixin Yu, Jiandong Bao, Li Zhang
Cell Signal. doi: 10.1016/j.cellsig.2020.109733. (IF 3.968)
73. Xiaoye Cheng, Zain-Ui Aabdin, Yan Wang, Nana Ma, Hongyu Dai, Xiaoli Shi, Xiangzhen Shen
J Dairy Sci. doi: 10.3168/jds.2020-18402. (IF 3.333)
74. J J Zhang, L J Cai, K Pang, Y Dong, Z G Zhang, B B Li, R Li, C H Han
Clin Transl Oncol. doi: 10.1007/s12094-020-02455-y. (IF 2.737)
75. Jingjing Zong, Hao Peng, Xin Qing, Zhe Fan, Wenjing Xu, Xuanlong Du, Ruihua Shi, Yewei Zhang
ACS Omega. doi: 10.1021/acsomega.1c01346. (IF 2.87)
76. Haiquan Zhu, Shaobo Zhu, Xiuchao Shang, Xiangsheng Meng, Sheng Jing, Li Yu, Yu Deng
Front Genet. doi: 10.3389/fgene.2021.648709. (IF 3.258)
77. Chunsen Li, Zhenzhen Li, Lingxie Song, Lian Meng, Guixuan Xu, Haijun Zhang, Jianming Hu, Feng Li, Chunxia Liu
Front Oncol. doi: 10.3389/fonc.2021.656608. (IF 4.848)
78. Hong Zhang, Susu Wang, Yaqian Wang, Anxin Lu, Chunping Hu, Chonghuai Yan
Mol Med Rep. doi: 10.3892/mmr.2021.12197. (IF 2.1)
79. Yu Gu, Weiming Liu, Gaoqin Liu, Xin Li, Peirong Lu
Mol Med Rep. doi: 10.3892/mmr.2021.12379. (IF 2.1)
80. Shouzhu Xu, Jie Xu, Ting Hao, Yu Yan, Shihao Zhang, Aihong Li, Chuandao Shi, Qiling Liu, Jing Zhao
Mol Med Rep. doi: 10.3892/mmr.2021.12419. (IF 2.1)
81. Junhao Zhu, Chao Tang, Zixiang Cong, Feng Yuan, Xiangming Cai, Jin Yang, Chiyuan Ma
Endocr Relat Cancer. doi: 10.1530/ERC-21-0215.
82. Meijuan Meng, Lairong Wang, Yan Wang, Nana Ma, Wan Xie, Guangjun Chang, Xiangzhen Shen
J Dairy Sci. doi: 10.3168/jds.2021-21187. (IF 3.333)
83. Zhao Yan, Yaxin Hu, Yiwei Zhang, Qian Pu, Liangzhao Chu, Jian Liu
Mol Clin Oncol. doi: 10.3892/mco.2022.2516.
84. Qihang Li, Zhiyu Zhang, HongChao Jiang, Jun Hou, Yuhang Chai, Hongxing Nan, Feng Li, Lianghai Wang
J Transl Med. doi: 10.1186/s12967-022-03449-w. (IF 4.124)
85. Yan Sun, Xiaobing Chen, Yongpeng Xie, Yanli Wang, Qian Zhang, Yu Lu, Xiaomin Li
Immun Inflamm Dis. doi: 10.1002/iid3.641.
86. Jing Ji, Zhen Zhang, Xingbei He, Gang Pan, Guanchu Li, Jinyu Lv, Yuxin Xu, Mengru Xie, Jing Feng, Weiling Wang, Bin Liu, Jinming Ma, Xiujun Wang
Anticancer Drugs. doi: 10.1097/CAD.0000000000001475.