QuickBlock™ Western封闭液
收藏
产品编号 P0252-500ml
数量
¥ 498.00
产品包装:
15ml 100ml 500ml
产品简介
使用说明
说明书下载
相关产品
相关论文
产品问答

碧云天生产的QuickBlock™ Western封闭液(QuickBlock™ Blocking Buffer for Western Blot)是最新一代的快速高效Western封闭液,总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉、酪蛋白(Casein)等的封闭液及国外同类产品,主要用于Western blot (WB)实验中PVDF膜或硝酸纤维素膜(nitrocellulose membrane, NC膜)的封闭,也可以用于Western实验中一抗或二抗的稀释。
QuickBlock™ Western封闭液快速高效。封闭时间通常仅需5-15分钟,并且和BSA、脱脂奶粉、酪蛋白等传统的Western封闭液以及国外同类的快速封闭液相比,显示出更强的信噪比(参考图1)。
QuickBlock™ Western封闭液封闭后背景极低。本封闭液不含血清和白蛋白,确保极高的信噪比。
QuickBlock™ Western封闭液兼容性好,兼容辣根过氧化物酶(Horseradish peroxidase, HRP)、碱性磷酸酶(Alkaline
phosphatase, AP)和生物素标记的二抗。本产品中添加了不影响HRP和AP活性的防腐剂,不会干扰HRP或AP标记二抗的检测。同时本产品不含生物素,不会干扰基于生物素的检测。
QuickBlock™ Western封闭液使用便捷。本产品无需添加任何额外的试剂,可以直接用于印迹膜的封闭。
本产品与BSA及国外同类产品的封闭效果对比参见图1。在相同样品和实验条件下,仅封闭液及封闭时间存在如下图所示的差异时,碧云天的QuickBlock™ Western封闭液封闭后的整体背景明显低于BSA封闭后的背景,而且目的条带亮度明显高于国外同类品牌产品。
图1. QuickBlock™ Western封闭液与BSA及国外同类产品的封闭效果比较。每组实验从左到右依次为:5µl蛋白Marker,2.5µg蛋白量的HeLa细胞裂解液,5µg蛋白量的HeLa细胞裂解液。请注意对于Antibody A,QuickBlock™封闭液比BSA和国外同类产品具有更低的背景,并且和国外同类产品相比信号明显更强。对于Antibody B,QuickBlock™封闭液比BSA具有更低的背景,和国外同类产品相比信号明显更强。实际实验结果会因样品、抗体、实验条件等的不同而存在差异,图中数据仅供参考。

关于不同封闭液的比较和选择,请参考碧云天的相关网页:http://www.beyotime.com/support/blocking-buffer.htm
按照每张膜封闭需要5-10ml QuickBlock™ Western封闭液计算,一个100ml包装的本产品可以封闭10-20张膜,一个500ml包装的本产品可以封闭50-100张膜。
包装清单:

产品编号 产品名称 包装
P0252-100ml QuickBlock™ Western封闭液 100ml
P0252-500ml QuickBlock™ Western封闭液 500ml
说明书 1份

保存条件:
4℃保存,一年有效。长期不使用可以-20℃保存。
注意事项:
通常本产品用于PVDF膜及NC膜时的封闭时间为5-15分钟。对于一些背景非常高的抗体,可以尝试将封闭时间延长为30-60分钟。此外,如有特殊需要,也完全可以4℃封闭过夜。
由于没有任何一种封闭液是适用于所有实验体系的,因此对于一些特殊的实验,可能需要根据具体情况考虑使用其它更合适的封闭液。
取放PVDF膜和NC膜应使用平头镊子,并仅轻轻夹取其边角,操作过程须避免膜表面产生划痕、折痕或压痕等痕迹。
PVDF膜一经浸润和活化,需一直保持湿润,根据Western进行到的具体步骤可放置于western转膜液或洗涤液等适当溶液中,否则可能会产生难以封闭的异常背景。
为进一步提高信噪比,推荐同时使用QuickBlock™Western一抗稀释液(P0256)QuickBlock™ Western二抗稀释液(P0258)进行一抗及二抗的稀释。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 完成转膜后,用Western洗涤液洗涤印迹膜1-2分钟。
2. 根据膜的大小,在平皿或者其它适当容器中倒入一定体积的QuickBlock™ Western封闭液,确保封闭液后续能充分覆盖膜即可。对于常规的western,一张约6.6×8.5cm的膜推荐使用约10ml左右的封闭液。
3. 用平头镊夹住膜的一角,将膜放置在QuickBlock™ Western封闭液中,使封闭液完全浸没膜,置于水平摇床上封闭约10分钟(通常5-15分钟均可;经多种抗体的测试封闭10分钟的效果很多时候会显著优于常规的BSA封闭1小时的效果)。
4. 封闭后的膜即可用于一抗孵育等后续实验。详细的Western操作可以参考如下的相关网页:
http://www.beyotime.com/support/western.htm

相关产品:

产品编号 产品名称 包装
P0023A-100ml Western一抗稀释液 100ml
P0023A-500ml Western一抗稀释液 500ml
P0023B-100ml Western封闭液 100ml
P0023B-500ml Western封闭液 500ml
P0023D-100ml Western二抗稀释液 100ml
P0023D-500ml Western二抗稀释液 500ml
P0220 QuickBlock™封闭液(PBS) 100ml
P0222 QuickBlock™封闭液(PBSTw)  100ml
P0226 QuickBlock™封闭液(PBSTx) 100ml
P0228 QuickBlock™封闭液(TBS) 100ml
P0231 QuickBlock™封闭液(TBSTw) 100ml
P0233 QuickBlock™封闭液(TBSTx)  100ml
P0235 QuickBlock™封闭液(10X)  100ml
P0252-100ml QuickBlock™ Western封闭液 100ml
P0252-500ml QuickBlock™ Western封闭液 500ml
P0256-100ml QuickBlock™ Western一抗稀释液 100ml
P0256-500ml QuickBlock™ Western一抗稀释液 500ml
P0258-100ml QuickBlock™ Western二抗稀释液 100ml
P0258-500ml QuickBlock™ Western二抗稀释液 500ml


相关产品请点击如下按钮:
使用本产品的文献:
2. Chen J, Mao S, Li H, Zheng M, Yi L, Lin JM, Lin ZX.
PLoS One . 2017 Aug 3;12(8):e0182183. (IF 2.74)
3. Chen GL, Ye T1, Chen HL3, Zhao ZY4, Tang WQ4, Wang LS3, Xia JL1,4.
Oncogenesis . 2017 Sep 25;6(9):e382. (IF 6.119)
5. Ding Y, Zheng Y, Liu T, Chen T, Wang C, Sun Q, Hua M, Hua T.
SCI REP-UK . 2017 Nov 2;7(1):14897. (IF 3.998)
8. Tengfei Liu, Xiaoyan Liu, Hui Xiong, Cheng Xu, Jianxu Yao, Xiumei Zhu, Jianping Zhou and Jing Yao.
POLYM CHEM-UK . 2018;9:1827-1839. (IF 5.342)
9. Ding M1,2,3, Ning J2, Feng N2, Li Z2, Liu Z2, Wang Y2, Wang Y2, Li X3, Huo C3, Jia X3, Xu R3, Fu F2, Wang X3, Pei J2.
J Pineal Res . 2018 Jan;64(1). doi: 10.1111/jpi.12447. (IF 14.528)
10. Yang D1, Sun C1, Zhang J2, Lin S2, Zhao L2, Wang L1, Lin R1, Lv J1, Xin S1.
Int J Mol Med . 2018 Jan;41(1):43-50. (IF 3.098)
11. Liu X1, Wang Z2, Zhang G3, Zhu Q3, Zeng H3, Wang T3, Gao F3, Qi Z3, Zhang J4, Wang R3.
Oncol Lett . 2018 Jan;15(1):1229-1235. (IF 2.311)
12. Zhang L,Liu J,Zhou F,Wang W,Chen N
Mol Med Rep. 2018 Mar;17(3):4490-4498. (IF 2.1)
13. Wang X,Zou P,He Y,Meng K,Quan F,Zhang Y
Anim Reprod Sci. 2018 Mar;190:108-118. (IF 1.66)
14. Liu L,Chang X,Zhang Y,Wu C,Li R,Tang L,Zhou Z
Toxicol In Vitro. 2018 Mar;47:228-237. (IF 2.959)
15. Chen G1, Li Q1, Luo Y2, Liu T2, Zhou S1, Pan E3, Peng L3.
MOL MED REP . 2018 Apr;17(4):5928-5933. (IF 2.1)
16. Zhao Z1, Zhao Y1, Zhuang XY2, Lo WC3, Baker MAB4, Lo CJ5, Bai F
Nature Communications . 2018 May 14;9(1):1885. (IF 11.878)
17. Guo D1, Hu X2, Zhang H3, Lu C4, Cui G5, Luo X6.
Int Immunopharmacol . 2018 May;58:72-79. (IF 3.943)
18. Song Z1, Yang F1, Du H1, Li X1, Liu J1, Dong M1, Xu X2,3.
Thorac Cancer . 2018 May;9(5):555-562. (IF 2.61)
19. Xu H,Chen J,Si X,Chen M,Pei F,Qiu C,Wu J,Guan X
Mol Med Rep. 2018 Jun;17(6):8548-8556. (IF 2.1)
20. Yang HW1, Liu XY2, Shen ZF1, Yao W1, Gong XB2, Huang HX3, Ding GH1.
Int J Biol Sci . 2018 Jun 22;14(9):1142-1152. (IF 4.858)
21. Sun H1, Shao W2, Liu H2, Jiang Z3.
ENVIRON SCI POLLUT R . 2018 Jun;25(17):17050-17057. (IF 3.056)
22. Gao S1, Wang Y2, Li D1, Guo Y1, Zhu M3, Xu S1, Mao J1, Fan G4.
Inflammation . 2018 Sep 14. doi: 10.1007/s10753-018-0891-7. (IF 3.212)
23. Liu L1, Zhang Y1, Chang X1, Li R2, Wu C1, Tang L3, Zhou Z4.
Toxicol Lett . 2018 Oct 1;295:277-287. (IF 3.569)
24. Wang Z,Zhou W,Dong H,Ma X,He Z
Mol Med Rep. 2018 Oct;18(4):3957-3964. (IF 2.1)
25. Jin X1, Yu Y1, Zou Q2, Wang M1, Cui Y1, Xie J1, Wang Z1.
J Cell Biochem . 2018 Oct 14. doi: 10.1002/jcb.27873. (IF 4.237)
26. Hu H1, Zhao P2, Liu J2, Ke Q2, Zhang C1, Guo Y3, Ding H4.
J NANOBIOTECHNOL . 2018 Nov 29;16(1):98. (IF 6.518)
28. Lin L,Wang L,Liu Y,Xu C,Tu Y,Zhou J
Oncol Rep. 2018 Dec;40(6):3405-3415. (IF 3.417)
29. Wu K,Mu XY,Jiang JT,Tan MY,Wang RJ,Zhou WJ,Wang X,He YY,Li MQ,Liu ZH
Oncol Rep. 2018 Dec;40(6):3523-3532. (IF 3.417)
30. Wu JX,Tong L,Hu L,Xia CM,Li M,Chen QH,Chen FX,Du DS
Hypertens Res. 2018 Dec;41(12):1013-1022. (IF 2.941)
31. Ding Y1,2, Chen T1, Wang Q1, Yuan Y1, Hua T1.
Neuroreport . 2018 Dec 12;29(18):1537-1543. (IF 1.394)
33. Liang X, Li B, Huang Q, Liu D, Ma H.
BIOCHEM BIOPH RES CO . 2018 Dec 9;507(1-4):355-361. (IF 2.985)
34. Li XH1, Wang HP1, Tan J1, Wu YD1, Yang M1, Mao CZ2, Gao SF2, Li H2, Chen H3, Cai WB4.
Life Sci . 2019 Jan 1;216:129-139. (IF 3.647)
35. Wang X,Meng K,He Y,Wang H,Zhang Y,Quan F
Int J Biol Sci. 15(2):404-415. (IF 4.858)
37. Pei-PeiZhao, Hao-RanHu, Jia-YuLiu, Qin-FeiKe, Xiao-YuanPeng, HaoDing, Ya-PingGuo.
CHEM ENG J . 2019 March 1;359: 1120-1129. (IF 10.652)
38. Xiao Q, Zeng JH, Zhou H, Qiu QH, Ke B, Deng L, Hu ZM, Roh J, Dai M.
Mol Med Rep. 19(3):2377-2385. (IF 2.1)
39. Li M,Wang J,Liu Y,Wu J,Li F,Feng S,Du D,Chen F
Neuroreport. 30(9):637-644. (IF 1.394)
40. Pan Z,Fan Z,Ma J,Liu H,Shen L,He B,Zhang M
Am J Transl Res. 11(6):3801-3815. eCollection 2019. (IF 3.375)
42. Deng Y,Han Q,Mei S,Li H,Yang F,Wang J,Ge S,Jing X,Xu H,Zhang T
Oncol Lett. 18(3):2845-2852. (IF 2.311)
43. Tan Y,Jin Y,Wang Q,Huang J,Wu X,Ren Z
Cells. 8(10). pii: E1241. (IF 4.366)
45. Wang J,Feng S,Li M,Liu Y,Yan J,Tang Y,Du D,Chen F
Neurochem Res. 44(12):2796-2808. (IF 3.038)
46. Feng Min,Xian Jie Jia,Qin Gao,Fang Niu,Zhi Yuan Hu,Ya Ling Han,Hong Jie Shi,Ying Yu
Exp Ther Med. doi: 10.3892/etm.2019.8176. (IF 1.785)
48. Jiangbei Yuan,Zihan Zheng,Liting Wang,Haiying Ran,Xiangyu Tang,Xiaodong Xie,Fei Li,Fang Liu,Xiaoyang Wang,Jiale Zhang,Junying Zhang,Yi Huang,Xuefeng Xia,Ying Wan
Infect Immun. doi: 10.1128/IAI.00738-19. (IF 3.201)
49. Yang X,Zhang C,Tie H,Luo J,Wang Y,Wu Q
J Cell Biochem. 121(4):2950-2961. (IF 4.237)
50. Feng-Zhi Xin,Ze-Hua Zhao,Rui-Nan Zhang,Qin Pan,Zi-Zhen Gong,Chao Sun,Jian-Gao Fan
World J Gastroenterol. doi: 10.3748/wjg.v26.i18.2203.
51. Xin Dang,Nai-Kei Wong,Yongli Xie,Vengatesen Thiyagarajan,Fan Mao,Xiangyu Zhang,Yue Lin,Zhiming Xiang,Jun Li,Shu Xiao,Zohaib Noor,Yuanqiu He,Yang Zhang,Ziniu Yu
Front Cell Dev Biol. doi: 10.3389/fcell.2020.00411. (IF 5.201)
52. Xiangru Zheng,Jinlai Wei,Wenjun Li,Xiaoli Li,Wuyi Wang,Jinbao Guo,Zhongxue Fu
Aging (Albany NY). doi: 10.18632/aging.103690.
53. Hui Hou,Ruirui Yang,Xiaohong Liu,Xiaolong Wu,Sulin Zhang,Kaixian Chen,Mingyue Zheng
Bioorg Chem. doi: 10.1016/j.bioorg.2020.103958. (IF 4.831)
54. Chufan Yan,Jiangyue Zhao,Yu Qin,Fangkun Zhao,Liyang Ji,Jinsong Zhang
Mol Med Rep. doi: 10.3892/mmr.2020.11205. (IF 2.1)
55. Niya Long,Shuo Peng,Liangzhao Chu,Jun Jia,Minghao Dong,Jian Liu
Exp Ther Med. doi: 10.3892/etm.2020.9200. (IF 1.785)
56. Yi Wu,Heming Cui,Yuying Zhang,Ping Yu,Yuangeng Li,Dan Wu,Yan Xue,Wenwen Fu
Mol Med Rep. doi: 10.3892/mmr.2020.11716.
57. Dongmei Ye,Yuxuan Li,Heliang Zhang,Zhiwei Zhou,Yujie Tang,Peng Wu,Qiang Zhao,Zhiwei Zhang
Int J Biol Sci. doi: 10.7150/ijbs.52591.
58. Donghua Du,Wenting Lv,Rina Su,Chunwei Yu,Xiaoxia Jing,Nuwenqimuge Bai,Surong Hasi
Cell Stress Chaperones. doi: 10.1007/s12192-020-01184-z.
59. Cai-Chou Zhao,Hao Guo,Ying Wang,Jiu-Hong Li
Hum Cell. doi: 10.1007/s13577-020-00473-0.
60. Jiechun Huang,Fangrui Wang,Xiaotian Sun,Xianglin Chu,Rongrong Jiang,Yiqing Wang,Liewen Pang
Am J Transl Res.
61. Shan-Shan Li,Ling-Ling Xie,Zhuang-Zhuang Li,Yong-Jian Fan,Man-Man Qi,Yan-Guo Xi
Neurosci Lett. doi: 10.1016/j.neulet.2021.135842.
62. Yanjie Tan,Yi Jin,Sheng Wang,Jianhua Cao,Zhuqing Ren
J Muscle Res Cell Motil. doi: 10.1007/s10974-020-09585-4.
63. Wenjin Chen,Weiqiang Jia,Cuiying Wu,Lihua Chen,Kai Sun,Ji Wang,Boyun Ding,Ning Liu,Ruxiang Xu
Front Oncol. doi: 10.3389/fonc.2021.644492.
64. Keke Wu,Biao Li,Qiuzhen Lin,Wanghan Xu,Wanyun Zuo,Jiayi Li,Na Liu,Tao Tu,Baojian Zhang,Yichao Xiao,Qiming Liu
Life Sci. doi: 10.1016/j.lfs.2021.119299.
65. Jingfei Zheng,Xuehe Li,Weili Yang,Fang Zhang
J Pharmacol Sci. doi: 10.1016/j.jphs.2021.02.001.
66. Bin Zeng,Zhiwei Sun,Qiting Zhao,Doudou Liu,Hao Chen,Xiaoshuang Li,H Rosie Xing,Jianyu Wang
Int J Biol Sci. doi: 10.7150/ijbs.60866.
67. Li-Min Zhang,Dong-Xue Zhang,Wei-Chao Zheng,Jin-Shu Hu,Lan Fu,Yan Li,Yue Xin,Xu-Peng Wang
Exp Neurol. doi: 10.1016/j.expneurol.2021.113683.
68. Chao Zhou,Mengyu Liu,Xiang Mei,Qian Li,Wenjuan Zhang,Ping Deng,Zhixin He,Yu Xi,Tong Tong,Huifeng Pi,Yonghui Lu,Chunhai Chen,Lei Zhang,Zhengping Yu,Zhou Zhou,Mindi He
Sci Total Environ. doi: 10.1016/j.scitotenv.2021.147014.
69. Chuan-Suo Zhang,Qian Han,Zhao-Wei Song,Hong-Yan Jia,Tian-Peng Shao,Yan-Peng Chen
Exp Ther Med. doi: 10.3892/etm.2021.10268.
70. Jiaqi Chen,Qiaoya Pan,Yang Bai,Xuepeng Chen,Yi Zhou
Front Mol Biosci. doi: 10.3389/fmolb.2021.720370.
71. Guohai Xie,Xinyi Zheng,Zhong Zheng,Ruoyu Wu,Zhixian Yao,Wenjie Huang,Feng Sun,Xingyu Mu,Ke Wu,Junhua Zheng
Aging (Albany NY). doi: 10.18632/aging.203514.
72. Song Yu,Bingxuan Jia,Na Liu,Dianzhen Yu,Shuo Zhang,Aibo Wu
Sci Total Environ. doi: 10.1016/j.scitotenv.2021.147405.
73. Hang-Xing Yu,Wei Lin,Kang Yang,Li-Juan Wei,Jun-Li Chen,Xin-Yue Liu,Ke Zhong,Xin Chen,Ming Pei,Hong-Tao Yang
Front Pharmacol. doi: 10.3389/fphar.2021.741801.
74. Si-Min Zhang,Chuan-Yuan Wei,Qiang Wang,Lu Wang,Lu Lu,Fa-Zhi Qi
Mol Biol Rep. doi: 10.1007/s11033-021-06646-w.
75. Lishan Zhang,Shanhui Ge,Wanmei He,Qingui Chen,Caixia Xu,Mian Zeng
J Biol Chem. doi: 10.1016/j.jbc.2021.101111.
76. Qudong Lu,Yang Yang,Hengshuai Zhang,Cheng Chen,Jiang Zhao,Zhenxing Yang,Yi Fan,Longkun Li,Huan Feng,Jingzhen Zhu,Shanhong Yi
Drug Des Devel Ther. doi: 10.2147/DDDT.S329507.
77. Qian Zhou,Hong Cao,Xiaoyi Hang,Huamin Liang,Miaomiao Zhu,Yixian Fan,Jiawei Shi,Nianguo Dong,Ximiao He
Front Cell Dev Biol. doi: 10.3389/fcell.2021.794058.
78. Xin Wang,Wenting Zhang,Jingtao Na,Yanping Huo,Yacheng Wang,Ketong Liu
J Healthc Eng. doi: 10.1155/2021/3843830.
79. Yuhan Shu,Xin Sun,Guiqin Ye,Mengting Xu,Zhipan Wu,Caixia Wu,Shouxin Li,Jingkui Tian,Haote Han,Jianbin Zhang
Front Cell Dev Biol. doi: 10.3389/fcell.2021.760022.
80. Weibo Dai,Xinyi Zhan,Weijie Peng,Xin Liu,Weiwen Peng,Quanxi Mei,Xianjing Hu
Oxid Med Cell Longev. doi: 10.1155/2021/2617881.
81. Bin Wang,Ping Yu,Wei Lin,Zhaohui Zhai
Anal Cell Pathol (Amst). doi: 10.1155/2021/5543212.
82. Wenting Zhang,Xin Wang,Jing Li,Mingyuan Xu,Xiaolu Ren,Huiying Liu,Lu Xu,Jun Shao
J Healthc Eng. doi: 10.1155/2021/3433615.
83. Mengmeng Li,Hongjian Lu,Xueyan Wang,Chengwei Duan,Xiangyang Zhu,Yi Zhang,Xin Ge,Feng Ji,Xueqin Wang,Jianbin Su,Dongmei Zhang
Mol Immunol. doi: 10.1016/j.molimm.2021.10.017.
84. Li-Tian Ma,Yang Bai,Jie Li,Yu Qiao,Yang Liu,Jin Zheng
Chin J Integr Med. doi: 10.1007/s11655-021-3438-3.
85. Xiaoqin Tan,Chunpu Li,Ruirui Yang,Sen Zhao,Fei Li,Xutong Li,Lifan Chen,Xiaozhe Wan,Xiaohong Liu,Tianbiao Yang,Xiaochu Tong,Tingyang Xu,Rongrong Cui,Hualiang Jiang,Sulin Zhang,Hong Liu,Mingyue Zheng
J Med Chem. doi: 10.1021/acs.jmedchem.1c01205.
86. Yihan Wang,Xiang Yuan,Malik Ahsan Ali,Ziyue Qin,Yan Zhang,Changjun Zeng
Front Cell Dev Biol. doi: 10.3389/fcell.2021.792994.
87. Penghui Xu,Xing Zhang,Jiacheng Cao,Jing Yang,Zetian Chen,Weizhi Wang,Sen Wang,Lu Zhang,Li Xie,Lang Fang,Yiwen Xia,Zhe Xuan,Jialun Lv,Hao Xu,Zekuan Xu
Clin Transl Med. doi: 10.1002/ctm2.707.