Hoechst 33258染色液
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产品编号 ： C1017
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¥ 111.00 元
产品包装:
10ml 50ml
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Hoechst 33258，也称bisBenzimide H 33258或HOE 33258。分子式为C25H24N6O·3HCl，分子量为533.88，CASNumber 23491-45-4。
Hoechst 33258是一种可以穿透细胞膜的蓝色荧光染料，对细胞的毒性较低。
Hoechst 33258染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。Hoechst 33258也常用于普通的细胞核染色，或常规的DNA染色。
Hoechst 33258的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33258和双链DNA结合后，最大激发波长为352nm，最大发射波长为461nm。
本Hoechst 33258染色液可直接用于固定细胞或组织的细胞核染色，也可直接用于活细胞或组织的细胞核染色。
包装清单：

产品编号 产品名称 包装
C1017 Hoechst 33258染色液 10ml
— 说明书 1份

保存条件：
-20℃避光保存，一年有效。
注意事项：
本Hoechst 33258染色液的浓度经过碧云天的优化，确保可以满足各种常规染色的需要。如需使用特定浓度的Hoechst33258，请选购碧云天的Hoechst 33258(C1011)。如需染色活细胞或培养的组织，请选购碧云天的Hoechst 33342活细胞染色液(100X) (C1027、C1028或C1029)，效果更佳。
荧光染料都存在淬灭的问题，建议染色后尽量当天完成检测。活细胞或组织染色后宜立即观察。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。
Hoechst 33258对人体有害，请注意适当防护。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 对于固定的细胞或组织：
a.对于细胞或组织样品，固定后，适当洗涤去除固定剂。随后如果需要进行免疫荧光染色，则先进行免疫荧光染色，染色完毕后再按后续步骤进行Hoechst 33258染色。如果不需要进行其它染色，则直接进行后续的Hoechst 33258染色。
b.对于贴壁细胞或组织切片，加入少量Hoechst 33258染色液，覆盖住样品即可。对于悬浮细胞，至少加入待染色样品体积3倍的染色液，混匀。室温放置3-5分钟。
c. 吸除Hoechst 33258染色液，用TBST、PBS或生理盐水洗涤2-3次，每次3-5分钟。
d.直接在荧光显微镜下观察或封片后荧光显微镜下观察。细胞发生凋亡时，会看到凋亡细胞的细胞核呈致密浓染，或呈碎块状致密浓染。
2. 对于活细胞或培养的组织：
a. 加入适当量Hoechst 33258染色液，必须充分覆盖住待染色的样品，通常对于六孔板一个孔需加入1ml 染色液，对于96孔板一个孔需加入100微升染色液。
b. 在适宜于细胞培养的温度培养20-30分钟。弃染色液，用PBS或培养液洗涤2-3次即可进行荧光检测。细胞发生凋亡时，在荧光显微镜下观察会看到凋亡细胞的细胞核呈致密浓染，或呈碎块状致密浓染。

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C1011
10mg
Hoechst 33258
C1027
0.1ml
Hoechst 33342活细胞染色液(100X)
C1025
10ml
Hoechst 33342染色液
C1022
10mg
Hoechst 33342
C1005
10ml
DAPI染色液
C0003
100次
细胞凋亡－Hoechst染色试剂盒
C1018
50ml
Hoechst 33258染色液
C1002
5mg/ml×0.2ml
DAPI
C1029
3ml
Hoechst 33342活细胞染色液(100X)
C1028
0.5ml
Hoechst 33342活细胞染色液(100X)
S0033S
>100次
活性氧检测试剂盒
C1026
50ml
Hoechst 33342染色液